The subcellular distribution of alanine-glyoxylate aminotransferase and serine-pyruvate aminotransferase in dog liver
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چکیده
منابع مشابه
The subcellular distribution of rat liver L-alanine-glyoxylate aminotransferase in relation to a pathway for glucose formation involving glyoxylate.
1. The distribution of l-alanine-glyoxylate aminotransferase activity between subcellular fractions prepared from rat liver homogenates was investigated. The greater part of the homogenate activity (about 80%) was recovered in the ;total-particles' fraction sedimented by high-speed centrifugation and the remainder in the cytosol fraction. 2. Subfractionation of the particles by differential sed...
متن کاملIncreased liver L-serine-pyruvate aminotransferase activity under gluconeogenic conditions.
Rat liver l-serine-pyruvate aminotransferase activity exceeds markedly the normal adult value (a) in the neonatal period, (b) after glucagon injection and (c) after alloxan injection, observations that reinforce the suggestion from comparative findings that the aminotransferase has a role in gluconeogenesis. Some findings, however, argue in favour of l-serine dehydratase as the enzyme of glucon...
متن کاملCharacterization of peroxisomal targeting signals on alanine: glyoxylate aminotransferase.
Proteins destined for the peroxisome matrix or membrane possess distinct targeting signals that engage signal sequence receptors to drive their transport to their final subcellular destination. Peroxisomal targeting signal 1 (PTS1) and 2 (PTS2) are well characterized as signaling the import from the cytosol into the peroxisomal matrix of soluble peroxisomal enzymes. The expression vectors of th...
متن کاملIsolation and characterization of an L-alanine: glyoxylate aminotransferase from human liver.
L-Alanine:glyoxylate aminotransferase was isolated from human liver and purified 900-fold. Pyridoxal phosphate was required for catalytic activity and enhanced the stability of the enzyme during purification and storage. The enzyme underwent activation when heated for 10 min in 10 PM pyridoxal phosphate. Activation resulted in the formation of a nondialyzable pyridoxal phosphate complex with th...
متن کاملAlanine Aminotransferase
An examination of the subcellular distribution of alanine aminotransferase activity in pig cardiac tissue showed that about 10% of the total activity was bound to particulate material, with the highest specific activity in the sarcosomal fraction. The soluble enzyme was obtained in a high state of purity, as indicated by sedimentation velocity, starch gel electrophoresis, and spectral analyses....
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1979
ISSN: 0264-6021
DOI: 10.1042/bj1820877